LIPID-PEROXIDATION IN ISOLATED MEMBRANES OF CEREBRAL-CORTEX, HEART AND KIDNEY

The extent of ADP.Fe/NADPH-induced lipid peroxidation measured as prod uction of thiobarbituric acid-reactive substances (TBARS) was determin ed in isolated membranes from cerebral cortex, heart and kidney of 21- days-old rats. The time course of lipid peroxidation showed higher pro duction of TBARS in cerebral cortex than in heart and kidney. Our data indicate that high level of TBARS production is not due to high activ ity of NADPH oxidoreductase but due to high content of endogenous lipi ds in cerebral cortex membranes that could be modified. Higher product ion of TBARS in cerebral cortex is the result of higher content of lip ids in cerebral cortex membranes because NADPH cytochrome c reductase activity in membranes of cerebral cortex is lower than that of heart a nd kidney.