CAPILLARY ELECTROPHORESIS OF BIOLOGICALLY IMPORTANT COMPOUNDS - OPTIMIZATION OF SEPARATION CONDITIONS BY THE OVERLAPPING RESOLUTION MAPPINGSCHEME

The use of a simple and rapid systematic optimization scheme is descri bed. This scheme, known as the overlapping resolution mapping scheme ( ORM), makes use of nine preliminary experiments to predict the optimum separation conditions. In this investigation, the validity of the ORM scheme is verified by choosing the optimum separation conditions pred icted by the scheme in the separation of a group of twelve amino acids derivatised with the N-t-butoxycarbonyl functional group, and a group of seven anti-malarial drugs. The effect of the buffer composition on the separation is also discussed.