THE EFFECT OF COLUMN TEMPERATURE ON THE MIGRATION TIME OF PEPTIDES INFREE-SOLUTION CAPILLARY ELECTROPHORESIS

Authors
ZHANG YK CHEN N WANG L
Citation
Yk. Zhang et al., THE EFFECT OF COLUMN TEMPERATURE ON THE MIGRATION TIME OF PEPTIDES INFREE-SOLUTION CAPILLARY ELECTROPHORESIS, Journal of liquid chromatography, 16(17), 1993, pp. 3689-3697
Citations number
8
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
16
Issue
17
Year of publication
1993
Pages
3689 - 3697
Database
ISI
SICI code
0148-3919(1993)16:17<3689:TEOCTO>2.0.ZU;2-F
Abstract
The effect of column temperature on the migration times of peptides in free - solution capillary electrophoresis (FSCE) is discussed. It has been found that there are parallel linear relationships between the l ogarithm of the migration time and the reciprocal of column temperatur e. The changes in migration time with temperature for the peptides are predominantly due to the temperature - induced viscosity changes of w ater. The electrophoretic selectivity remains constant for a specific pair of peptides despite a considerable variation in the migration tim e for, each of the peptides in FSCE.