Looking for possible signs of vocal fatigue, acoustic waveform perturb
ation was measured in normal female subjects during sustained phonatio
n at various fundamental frequencies (F(o)). At none of the pitch leve
ls a rise of the jitter or the shimmer was found after 25 min of vocal
ization. On the other hand, an effect of F(o) was seen from the start:
above the habitual speaking F(o) of our subjects' voices there was a
tendency for the jitter to be higher and for the shimmer to be lower.