IMPROVING TRANSFORMATION METHOD FOR INDUSTRIAL YEASTS - CONSTRUCTION OF ADH1-APT2 GENE AND USING ELECTROPORATION

Strong expression system of yeast dominant selection marker gene was c onstructed for transformation of industrial yeasts. Bacterial aminogly coside phosphotransferase II (APT2) gene from Escherichia coli transpo son Tn903 was inserted between a yeast alcohol dehydrogenase I (ADH1) gene promoter and cytochrome c1 (CYC1) gene terminator. This ADH1-APT2 gene was strongly expressed in the transformed yeasts even in the cas e of integration type plasmid. Using this strong expressive dominant s election marker gene, five strains in nine industrial yeasts which wer e tested could not be transformed by lithium acetate method, but these industrial yeasts could be transformed by electroporation.