SUBCLONING AND RESTRICTION ENZYME MAPPING OF BOVINE ADENOVIRUS TYPE-2

Bovine adenoviruses (BAVs), causing both respiratory and/or enteral di seases in cattle, have been isolated in many countries all over the wo rld. In this study we report on the molecular cloning of the internal EcoRI fragments spanning 20.6-90.5%, and the internal SalI fragments s panning 3.1-65.2% of the BAV type 2 (BAV2; strain No. 19) genome into the plasmid vector pUC19. Moreover, the subcloning of the BAV2 genome facilitated the construction of a detailed physical restriction endonu clease map for BamHI, ClaI, EcoRI, HindIII, KpnI, NotI, NspV, PstI, Pv uI, SalI, XbaI and XhoI.