AN INVENTORY OF 1152 EXPRESSED SEQUENCE TAGS OBTAINED BY PARTIAL SEQUENCING OF CDNAS FROM ARABIDOPSIS-THALIANA

As part of the goal to generate a detailed transcript map for Arabidop sis thaliana, 1152 single run sequences (expressed sequence tags or ES Ts) have been determined from cDNA clones taken at random in libraries prepared from different sources of plant material: developing silique s, etiolated seedlings, flower buds, and cultured cells. Eight hundred and ninety-five different genes could be identified, 32% of which sho wed significant similarity to existing sequences in Arabidopsis and an array of other organisms. These sequences in combination with their p ositioning on the Arabidopsis genetic map will not only constitute a n ew set of molecular markers for genome analysis in Arabidopsis but als o provide a direct route for the in vivo analysis of their gene produc ts. The sequences have been made available to the public databases.