Lj. Robinson et al., MITOGEN-ACTIVATED PROTEIN-KINASE ACTIVATION IS NOT SUFFICIENT FOR STIMULATION OF GLUCOSE-TRANSPORT OR GLYCOGEN-SYNTHASE IN 3T3-L1 ADIPOCYTES, The Journal of biological chemistry, 268(35), 1993, pp. 26422-26427
The role of mitogen-activated protein (MAP) kinase in the regulation o
f glucose metabolism has been investigated by comparing the effects of
insulin and epidermal growth factor (EGF) on MAP kinase activation, g
lucose transport, and glycogen synthase in 3T3-L1 adipocytes. Insulin
or EGF treatment for 5 min increased p42mapk and p44mapk activity to t
he same extent as determined by myelin basic protein kinase activity m
easurements and phosphotyrosine immunoblotting. The profiles of myelin
basic protein kinase activity following MonoQ chromatography of extra
cts obtained from cells incubated with insulin or EGF were almost iden
tical. Insulin increased glucose transport and GLUT4 translocation to
the cell surface by 15- and 7-fold, respectively. EGF had no significa
nt effect on these processes. Insulin increased the glycogen synthase
ratio (-Glc-6-P/+Glc-6-P) by 7.5- and 3.5-fold in the presence and abs
ence of glucose, respectively. EGF increased the ratios by only 2- and
1.3-fold, respectively. EGF did not appear to inhibit downstream of M
AP kinase, because when adipocytes were incubated with insulin plus EG
F, the stimulation of glucose transport and glycogen synthase was simi
lar to that observed with insulin alone. These findings indicate that
activation of the MAP kinase isoforms p42mapk and p44mapk is not suffi
cient for the activation of glucose transport and glycogen synthase in
3T3-L1 adipocytes.