HEPARIN-BINDING PEPTIDES FROM THROMBOSPONDIN-1 AND THROMBOSPONDIN-2 CONTAIN FOCAL ADHESION-LABILIZING ACTIVITY

Citation
Je. Murphyullrich et al., HEPARIN-BINDING PEPTIDES FROM THROMBOSPONDIN-1 AND THROMBOSPONDIN-2 CONTAIN FOCAL ADHESION-LABILIZING ACTIVITY, The Journal of biological chemistry, 268(35), 1993, pp. 26784-26789
Citations number
45
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
268
Issue
35
Year of publication
1993
Pages
26784 - 26789
Database
ISI
SICI code
0021-9258(1993)268:35<26784:HPFTAT>2.0.ZU;2-8
Abstract
The cell adhesion regulating extracellular matrix glycoprotein, thromb ospondin (TSP), causes a loss of focal adhesion plaques from spread en dothelial cells and fibroblasts. To localize the site on TSP that has focal adhesion-labilizing activity, we initially tested proteolytic fr agments of TSP for activity. The heparin-binding fragment has signific ant focal adhesion-labilizing activity, whereas the nonheparin-binding 140-kDa fragment had no significant activity. These results were cons istent with previous data that showed that both a monoclonal antibody to the heparin-binding domain of TSP (A2.5) and heparin neutralized TS P activity. Peptides from putative heparin binding sequences of the am ino-terminal heparin-binding domain of TSP were synthesized and tested for their ability to cause loss of focal adhesions. The hep I peptide (amino acids 17-35) caused maximal loss of focal adhesions and was ac tive at 0.1 muM, whereas peptide hep II (74-95) and peptide hep III (1 70-189) were inactive. The activity of the hep I peptide was neutraliz ed by the addition of heparin and heparan sulfate but not by chondroit in sulfate. The basic amino acids in the hep I sequence appear to be r equired for focal adhesion-labilizing activity, because modification o f the lysine residues at amino acids 24 and 32 rendered the peptide co mpletely inactive. In addition, a peptide from the analogous sequence of mouse TSP 2, in which basic residues are conserved, was nearly as a ctive as hep I from TSP1. These data show that the anti-adhesive activ ity of TSP is conserved in both TSP1 and TSP2 and that the active site is located in a 19-amino acid sequence in the heparin-binding domain of TSPs.