SEQUENCE AND ARRANGEMENT OF 2 GENES OF THE BUTYRATE-SYNTHESIS PATHWAYOF CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824

Authors
WALTER KA NAIR RV CARY JW BENNETT GN PAPOUTSAKIS ET
Citation
Ka. Walter et al., SEQUENCE AND ARRANGEMENT OF 2 GENES OF THE BUTYRATE-SYNTHESIS PATHWAYOF CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824, Gene, 134(1), 1993, pp. 107-111
Citations number
15
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
134
Issue
1
Year of publication
1993
Pages
107 - 111
Database
ISI
SICI code
0378-1119(1993)134:1<107:SAAO2G>2.0.ZU;2-7
Abstract
The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate s ynthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate ki nase (buk), were sequenced. The genes are immediately adjacent on the chromosome, with ptb preceding buk. A single transcription start point (tsp) was identified 57 bp upstream from the ptb start codon by prime r extension analysis. The ptb and buk genes appear to form an operon. A putative Rho-independent terminator structure was identified 26 bp d ownstream from buk.