H. Synkova et M. Zajicova, PHOTOSYNTHETIC CHARACTERISTICS OF SOLANUM-TUBEROSUM L PLANTS TRANSFORMED BY AGROBACTERIUM STRAINS .2. PHOTOSYNTHETIC ACTIVITIES, Photosynthetica, 28(4), 1993, pp. 531-540
Photosynthetic and growth characteristics of the control potato plants
cv. Zvikov and those transformed by Agrobacterium were compared durin
g their cultivation in vitro in agar medium with 1% saccharose, after
having been transplanted into pots with soil, or growing from tubers i
n soil. The average leaf area, fresh and dry matter, chlorophyll a and
b ratio, and contents calculated per fresh and dry matter of the leaf
area were significantly higher in the control plants raised from tube
rs and in vitro cultivated than in the transplanted ones. The signific
ant increase in oxygen evolution by leaf fragments was found only in t
he control potato plants raised from tubers. Differences between photo
chemical activities of chloroplasts isolated from control and transfor
med plants were statistically significant only when calculated per fre
sh leaf matter. Chloroplasts from transformed potato plants grown from
tubers and from those cultivated in vitro exhibited higher activities
of photosystems (PS) 2 and PS 1 independently on the donors and accep
ters of electrons used. On the contrary, higher activities of both pho
tosystems were found in chloroplasts isolated from the control plants
transferred to soil.