THE COMPLETE AMINO-ACID-SEQUENCE OF CHITINASE-C FROM THE SEEDS OF RYE(SECALE-CEREAL)

The complete amino acid sequence of rye seed chitinase-c (RSC-c) has b een analyzed. This was done by first sequencing the tryptic peptides f rom RCm-RSC-c and then connecting them by analyzing the peptides produ ced by digestions with lysylendopeptidase and Staphylococcus aureas V8 protease of RCm-RSC-c, and by chymotryptic digestion and formic acid cleavage of S. aureus V8 protease peptides. RSC-c consists of 243 amin o acid residues and has a molecular mass of 26,093, and has 92% sequen ce homology with barley seed basic chitinase which lacks a Cys-rich do main. Cys204 is free and six cysteine residues are linked by disulfide bonds between Cys23 and Cys85, Cys97 and Cys105, and Cys223 and Cys23 6.