PREPARATION OF 3'-PHOSPHOADENOSINE-5'-PHOSPHO S-35 SULFATE USING ATP SULFURYLASE AND APS KINASE FROM BACILLUS-STEAROTHERMOPHILUS - ENZYMATIC-SYNTHESIS AND PURIFICATION

Authors
HIRAM P FERNANDO P KARAKAWA A SAKAKIBARA Y IBUKI H NAKAJIMA H LIU MC SUIKO M
Citation
P. Hiram et al., PREPARATION OF 3'-PHOSPHOADENOSINE-5'-PHOSPHO S-35 SULFATE USING ATP SULFURYLASE AND APS KINASE FROM BACILLUS-STEAROTHERMOPHILUS - ENZYMATIC-SYNTHESIS AND PURIFICATION, Bioscience, biotechnology, and biochemistry, 57(11), 1993, pp. 1974-1975
Citations number
7
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
11
Year of publication
1993
Pages
1974 - 1975
Database
ISI
SICI code
0916-8451(1993)57:11<1974:PO3SSU>2.0.ZU;2-6
Abstract
Radioactive PAPS was synthesized with a predetermined specific activit y using enzymes purified from Bacillus stearothermophilus. The reactio n system, incorporating 1.5 mCi of S-35-H2SO4 and 500 nmol of ATP, pro duced 582 muCi of S-35-PAPS. The synthesized S-35-PAPS was purified by ECTEOLA cellulose chromatography, followed by desalting with DOWEX 50 W-X8. The final yield was 206 mumol of S-35-PAPS with a radioactivity of 411 muCi.