RESOLUTION ENHANCED NMR-SPECTROSCOPY IN BIOLOGICAL-SYSTEMS VIA MAGNETIC-SUSCEPTIBILITY MATCHED SAMPLE IMMERSION CHAMBERS

A technique is described which reduced the magnetic susceptibility ind uced line broadening in NMR spectra obtained from three biological sys tems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leu kemic myeloblasts yielded minimum linewidths of 1.3 Hz at 200 MHz(0.00 65 ppm) after 10 min of automated shimming. P-31 spectra from an in vi vo murine MCa mammary carcinoma yielded a well-resolved phosphorylchol ine resonance without proton decoupling and with the resistive shim co ll currents set to zero. P-31 spectra from a perfused suspension of RI F-1 fibrosarcoma cells exhibited a gamma-nucleoside triphosphate reson ance which was resolved into purine and pyrimidine components.