K. Endo et al., 2 CHRONIC MYELOGENOUS LEUKEMIA-CELL LINES WHICH REPRESENT DIFFERENT STAGES OF ERYTHROID-DIFFERENTIATION, British Journal of Haematology, 85(4), 1993, pp. 653-662
We established two cell lines, YN-1 and Y-1K, from the peripheral bloo
d of two chronic myclogenous leukaemia patients in blastic crisis. Cha
racterization of the YN-1 and Y-1K cells revealed that these cells exp
ressed erythroid lineage markers. However, there was a marked differen
ce in the level of gamma-globin mRNA and haemoglobin in YN-1 and Y-1K
cells. YN-1 contained approximately 1-5% benzidine-positive staining c
ells, whereas no benzidine-positive cells were observed in Y-1K cells.
Haemoglobin production in YN-1 cells was markedly increased with vari
ous chemical inducers of erythroid differentiation, but was not in Y-1
K cells. In contrast, Y-1K cells expressed CD34 stem cell antigen and
CD41 megakaryocyte-specific antigen. These observations suggested that
, although both cell lines were committed to the erythroid lineage, ea
ch cell line represented a distinct differentiation stage in the eryth
roid differentiation programme. Y-1K seemed to correspond to an early
stage of cells in erythroid lineage, whereas YN-1 represented a more a
dvanced stage in human erythropoiesis.