MINIMAL REQUIREMENTS FOR EXPONENTIAL-GROWTH OF LACTOCOCCUS-LACTIS

Citation
Pr. Jensen et K. Hammer, MINIMAL REQUIREMENTS FOR EXPONENTIAL-GROWTH OF LACTOCOCCUS-LACTIS, Applied and environmental microbiology, 59(12), 1993, pp. 4363-4366
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
59
Issue
12
Year of publication
1993
Pages
4363 - 4366
Database
ISI
SICI code
0099-2240(1993)59:12<4363:MRFEOL>2.0.ZU;2-H
Abstract
A minimal growth medium containing glucose, acetate, vitamins, and eig ht amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h-1. With 19 amino acids added, the growth rate increased to mu = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suit able buffer for L. lactis and may be applied in high concentrations.