Pr. Jensen et K. Hammer, MINIMAL REQUIREMENTS FOR EXPONENTIAL-GROWTH OF LACTOCOCCUS-LACTIS, Applied and environmental microbiology, 59(12), 1993, pp. 4363-4366
A minimal growth medium containing glucose, acetate, vitamins, and eig
ht amino acids allowed for growth of Lactococcus lactis subsp. lactis,
with a specific growth rate in batch culture of mu = 0.3 h-1. With 19
amino acids added, the growth rate increased to mu = 0.7 h-1 and the
exponential growth phase proceeded until high cell concentrations were
reached. We show that morpholinepropanesulfonic acid (MOPS) is a suit
able buffer for L. lactis and may be applied in high concentrations.