T. Kometani et al., LARGE-SCALE PREPARATION OF (R)-1,2-PROPANEDIOL THROUGH BAKER YEAST-MEDIATED BIOREDUCTION, Journal of fermentation and bioengineering, 76(5), 1993, pp. 414-415
A procedure for the preparation of (R)-1,2-propanediol on a large scal
e by baker's yeast-mediated bioreduction of acetol using ethanol as an
energy source is reported. The bioreduction, catalyzed by NADH-depend
ent oxidoreductase, was effectively performed using a bubble-column re
actor with fed-batch operation. When the reactor with 10 l of medium w
as bubbled at an aeration rate of 3 l/min, the final reaction medium c
ontained 4 %(w/w) (R)-1,2-propanediol with >98% enantiomeric excess.