Dm. Kushlan et Dm. Lemaster, RESOLUTION AND SENSITIVITY ENHANCEMENT OF HETERONUCLEAR CORRELATION FOR METHYLENE RESONANCES VIA H-2-ENRICHMENT AND DECOUPLING, Journal of biomolecular NMR, 3(6), 1993, pp. 701-708
Monodeuterated methylene positions exhibit substantially superior spec
tral characteristics in H-1-C-13 correlation experiments as compared t
o diprotio signals. A combination of H-2 decoupling and multiplet edit
ing of HMQC and HSQC experiments provides resolution enhancement for b
oth stereoselective and random fractionally deuterated samples. For HM
QC experiments with [2-H-2(R),2-C-13]glycine-labeled E. coli thioredox
in (11.7 kDa), 3-fold increases in both H-1 and C-13 resolution result
in a complementary 9-fold enhancement in sensitivity. Owing to a smal
ler improvement in C-13 resolution, the corresponding enhancements for
the HSQC experiment are 2-fold less.