RESOLUTION AND SENSITIVITY ENHANCEMENT OF HETERONUCLEAR CORRELATION FOR METHYLENE RESONANCES VIA H-2-ENRICHMENT AND DECOUPLING

Monodeuterated methylene positions exhibit substantially superior spec tral characteristics in H-1-C-13 correlation experiments as compared t o diprotio signals. A combination of H-2 decoupling and multiplet edit ing of HMQC and HSQC experiments provides resolution enhancement for b oth stereoselective and random fractionally deuterated samples. For HM QC experiments with [2-H-2(R),2-C-13]glycine-labeled E. coli thioredox in (11.7 kDa), 3-fold increases in both H-1 and C-13 resolution result in a complementary 9-fold enhancement in sensitivity. Owing to a smal ler improvement in C-13 resolution, the corresponding enhancements for the HSQC experiment are 2-fold less.