DETERMINATION OF COMPATIBILITY IN MLC REA CTION FOR HEMOBLASTOSIS PATIENTS UNDER BONE-MARROW TRANSPLANTATION FROM HLA-IDENTICAL SIBS

Compatibility in mixed lymphocyte culture (MLC) test was determined fo r 60 hemoblastosis patients with their 71 HLA-identical sibs to transp lant allogenic syngeneic bone marrow. A protocol for the MLC radioisot ope micromethod with culturing in plates is described for the pair don or-recipient in the direct and reverse variants covering about 20 vari ants of mixed cultures. This secures the results' reliability. Plottin g of the MLC compatibility results for each pair has been proposed. In compatibility by MLC was revealed in 5 out of 71 pairs (7%). Bone marr ow transplantation was performed in 30 patients from their HLA-identic al/MLC-compatible sibs. The secondary disease due to incompatibility m issed in the MLC reaction occurred in 5 cases (16.7%), transplant reje ction in 2 cases (6,7%), which is less in number than literature data. It was established that determination of compatibility by MLC may ser ve a reliable criterion of assessing a genotypical pair HLA identity a nd should be conducted before each allogenic and syngeneic transplanta tion of bone marrow. The protocols of the method performance and compa tibility parameters evaluation warrant high significance of the compat ibility definition confirmed by clinical responses with minimal probab ility of secondary disease.