PLOIDY STUDIES BY IMAGE-ANALYSIS ON FINE-NEEDLE ASPIRATES OF THE BREAST

Quantitative DNA analysis was performed on preoperative fine needle as pirates of 12 breast cancers and 1 benign lesion using the Cell Analys is Systems (CAS) Model 200 image analysis system. The smears were prep ared on CAS slides, routinely processed for Papanicolaou staining, and then destained and restained with Feulgen stain after cytologic evalu ation. Four cases were DNA diploid, 3 were tetraploid, 1 was diploid/t etraploid, and 5 were aneuploid. When compared to analysis of samples from the resected tumor, both DNA index and S-phase fraction were cons tant.