A MACINTOSH(R) PROGRAM FOR RESULT ANALYSIS IN HLA GENOTYPING BY THE MODIFIED PCR-RFLP METHOD

Citation
P. Merel et al., A MACINTOSH(R) PROGRAM FOR RESULT ANALYSIS IN HLA GENOTYPING BY THE MODIFIED PCR-RFLP METHOD, BioTechniques, 15(6), 1993, pp. 1072-1075
Citations number
2
Categorie Soggetti
Biochemical Research Methods
Journal title
ISSN journal
07366205
Volume
15
Issue
6
Year of publication
1993
Pages
1072 - 1075
Database
ISI
SICI code
0736-6205(1993)15:6<1072:AMPFRA>2.0.ZU;2-5
Abstract
We describe in this report a computer program for easy interpretation of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) patterns generated during HLA Class II typing by the modifi ed PCR-RFLP method. HLA-DQA1, HLA-DQB1, HLA-DRB1 and HLA-DPB1 typing r esult analysis are thus greatly simplified. The program also allows vi deo capture and image storage, which are becoming a new standard in mo lecular biology laboratories.