ENHANCED PROLIFERATION OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS AND TUMOR-INFILTRATING LYMPHOCYTES IN A PLASMA-DERIVED CELL-CULTURE FLUID

Authors
TORRES AR BURGER R RICH A SPENDLOVE R WARREN RP
Citation
Ar. Torres et al., ENHANCED PROLIFERATION OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS AND TUMOR-INFILTRATING LYMPHOCYTES IN A PLASMA-DERIVED CELL-CULTURE FLUID, BioTechniques, 15(6), 1993, pp. 1106-1109
Citations number
26
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
15
Issue
6
Year of publication
1993
Pages
1106 - 1109
Database
ISI
SICI code
0736-6205(1993)15:6<1106:EPOPMA>2.0.ZU;2-L
Abstract
The proliferation and activation of murine and human T-lymphocytes in a high-protein lymphocyte culture fluid (LCF) is compared to that of c ells cultured in 10% fetal bovine serum (FBS). Tumor-infiltrating lymp hocytes (TIL) proliferate exponentially in the LCF for up to 46 days a nd generate cell numbers that are nearly 100000-fold greater than cell s cultured in FBS. This rapid growth of T cells in LCF could have an i mportant impact in adoptive immunotherapy and gene therapy since cell growth is a limiting factor in these technologies.