D. Georges et al., PLANT-REGENERATION FROM AGED-CALLUS OF THE WOODY ORNAMENTAL SPECIES LONICERA-JAPONICA CV HALLS PROLIFIC, Plant cell reports, 13(2), 1993, pp. 91-94
Callus cultures were initiated from in vitro grown leaf, stem and root
segments of Lonicera japonica ''Hall's Prolific'', on a medium contai
ning 10.7 muM alpha-naphthtylacetic acid and 2.7 muM benzyladenine, wh
ile media with 2,4-dichlorophenoxyacetic acid led to a rapid necrosis
of explants. Shoot regeneration from true-callus (i.e. without any par
t of the original explant) was achieved for the three different source
tissues within 12 weeks. The highest rate of regeneration was obtaine
d by using benzyladenine (4.4 to 44.4 muM) as the sole hormone in the
medium. The regenerated shoots were readily elongated and rooted on th
e same medium as used for multiplication, and plantlets were subsequen
tly transferred to greenhouse conditions, where nearly 100% of them we
re successfully acclimatized. This is the first example of plant regen
eration from aged (greater-than-or-equal-to 6 month-old) true-callus o
f a woody ornamental species.