ENTERIC VASCULAR ENDOTHELIAL RESPONSE TO BACTERIAL-ENDOTOXIN

The response of enteric vasculature to endotoxin was examined at the u ltrastructural level using a murine model of endotoxin-induced acute d iarrhoea. Morphological changes indicative of endothelial damage were evident as early as 15 minutes following endotoxin challenge. These ch anges, characterized by widening of intercellular spaces, increased mi crovillous projections and the appearance of stress fibres, preceded t he leucocytic response. Endothelial damage increased with time, being associated with progressive degenerative changes in the plasma membran e, cytoplasm and organelles, ultimately leading to desquamation. These latter changes were temporally associated with margination of neutrop hils and platelet adhesion to the denuded subendothelium. The venules were the primary site of these changes while the capillaries were the least affected. The arterioles were markedly constricted with minimal endothelial damage. These changes suggest that the enteric vascular en dothelium may be an important target organ, and the resultant endothel ial injury may have implications in host responses to endotoxin.