EFFECTS OF DIHYDROPYRIDINES ON ALDOSTERONE SECRETION IN ADRENAL CAPSULE PREPARATIONS FROM PREGNANT RATS

The primary aim of this study was to determine when sensitivity in the aldosterone response to extracellular potassium (K+) decreases during pregnancy. Second, it tested the hypothesis that calcium channel alte rations occur in the adrenal cortex during pregnancy. The decreased se nsitivity to K+, observed at 22 days of gestation, was not evident at 15 days and between 18 and 36 h postpartum. Increases in extracellular calcium concentration heightened sensitivity to K+ in adrenal capsule preparations derived fi om nonpregnant rats but had no effect in preg nant animals. The influence of nifedipine and BAY K 8644 (blocker and activator, respectively, of voltage-operated calcium channels) on the aldosterone response to K+ and to adrenocorticotropic hormone (ACTH) w as studied. Sensitivity to K+ in nonpregnant rats decreased in the pre sence of nifedipine and became similar to that in pregnant rats. Respo nses to ACTH were not affected by nifedipine. BAY K 8644 produced a la rger increase in sensitivity in adrenal capsule preparations from preg nant than fi om nonpregnant rats, leading to superposition of the two dose-response curves to K+. These results indicate that voltage-operat ed calcium channels involved in aldosterone secretion are functionally impaired during pregnancy.