A cladistic analysis of eleven freshwater gomphonemoid diatom taxa yie
lded three equally most parsimonious cladograms. The three cladograms
suggest Gomphoneis Cleve is non-monophyletic as originally and recentl
y circumscribed. Gomphoneis elegans and G. transsilvanica (Pant.) Kram
mer are more closely related to Gomphopleura Reichelt ex Tempere than
to other members of the genus. Gomphoneis geitleri Kociolek and Stoerm
er appears to represent a monotypic lineage. Among the taxa with diffe
rentiated apical pore fields Gomphonema kaznakowi Mereschkowsky appear
s to be primitively astigmate. The three cladograms vary with regard t
o systematic affinities among the Gomphoneis herculeana (Ehrenberg) Cl
eve group, Miocene gomphonemoid species from Idaho (USA), gomphonemoid
taxa from East Africa and Gomphonema sensu stricto. Despite variabili
ty in number of puncta rows per stria and presence of an axial plate i
n the Miocene species from North America, inclusion of these taxa in t
he analysis provided better resolution of relationships than if they w
ere excluded.