THERMAL-STABILITY OF PROTEIN SECONDARY STRUCTURE IN LANGMUIR-BLODGETT-FILMS

Authors
NICOLINI C EROKHIN V ANTOLINI F CATASTI P FACCI P
Citation
C. Nicolini et al., THERMAL-STABILITY OF PROTEIN SECONDARY STRUCTURE IN LANGMUIR-BLODGETT-FILMS, Biochimica et biophysica acta, 1158(3), 1993, pp. 273-278
Citations number
18
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1158
Issue
3
Year of publication
1993
Pages
273 - 278
Database
ISI
SICI code
0006-3002(1993)1158:3<273:TOPSSI>2.0.ZU;2-C
Abstract
The temperature dependence of the secondary structure of photosyntheti c reaction centres from Rhodobacter sphaeroides in solution and in Lan gmuir-Blodgett film was studied by circular dichroism. It was shown th at the secondary structure of the protein was not affected in Langmuir -Blodgett films by heating up to 200 degrees C, while in solution it w as completely lost at 55 degrees C. Molecular order rather than decrea sed hydration degree was held responsible.