S. Wang et al., ISOLATION AND CHARACTERIZATION OF A CELL-LINE FROM THE EPITHELIAL-CELLS OF THE HUMAN FETAL PANCREAS, Cell transplantation, 6(1), 1997, pp. 59-67
Pancreatic cell lines are useful for basic studies of pancreatic biolo
gy and for possible application to cell transplantation therapies for
diabetes. A retroviral vector expressing simian virus 40 (SV40) T anti
gen and H-ras(val12) was used to infect a monolayer culture of epithel
ial cells from an 18-wk human fetal pancreas, Infected cells gave rise
to a clonal epithelial cell line, designated TRM-1. This cell line ex
presses epithelial markers as well as glut2 and small amounts of insul
in and glucagon, TRM-1 is the first cell line to be generated from the
human fetal pancreas and also the first cell line derived directly fr
om the fetal pancreas of any species. The approach that we have used t
o develop TRM-1 should be applicable to isolating cell lines from othe
r stages of human pancreatic development. Copyright (C) 1997 Elsevier
Science, Inc.