NEW DESIGN OF A FLOW-THROUGH PERMEATION CELL FOR STUDYING IN-VITRO PERMEATION STUDIES ACROSS BIOLOGICAL-MEMBRANES

A newly designed diffusion cell has been developed for in vitro permea tion studies across biological or artificial membranes. The cell has s everal unique properties to make it suitable for transdermal drug deli very studies: (i) it is two-chambered, allowing application of a donor with a finite or infinite dose; (ii) it allows continuous flow-throug h permeation of the acceptor phase, maintaining sink conditions and al lowing automatic sampling to obtain an accurate permeation profile; (i ii) it features a spiral acceptor fluid flow pattern, ensuring equal t ransport along - and moisturization of - the membrane and minimizing t he influence of volume and flow rate on mass transfer by eliminating a ny stagnant domains in the acceptor compartment.