The sediment phase of the Venice lagoon within an area of shallow wate
r, the Palude della Rosa, was studied and three insoluble enzymatic ac
tivities (cellulase, phosphatase and urease) were found to be linked t
o the inorganic phase. These immobilized enzymes were more resistant t
o environmental changes, even extreme ones, compared to their soluble
counterparts. The evolution of their activity with pH, temperature and
seasonal variation was investigated. While pH related activities show
ed the usual behaviour, the resistance to temperature was extremely hi
gh and seasonal variation was dependent on immobilization. These enzym
atic activities could be used as a diagnostic factor for the ecosystem
, since their presence is related to the nature of the waste products.