Mh. Pei et al., DISTINCTION BETWEEN STEM-INFECTING AND LEAF-INFECTING FORMS OF MELAMPSORA RUST ON SALIX-VIMINALIS USING RAPD MARKERS, Mycological research, 101, 1997, pp. 7-10
The RAPD technique was used to distinguish two forms of Melampsora rus
t on Salix viminalis. Fifteen isolates of the stem-infecting form and
19 of the leaf-infecting form collected from the U.K. were tested with
10 arbitrary primers. All the primers generated polymorphic bands and
46 putative loci were examined. Seven primers gave a total of eight b
ands specific for the stem-infecting form and four primers produced fi
ve bands characteristic of the leaf-infecting form. Cluster analysis u
sing Unweighted Pair-Group Method with Averaging (UPGMA) revealed 62%
disagreement between the two forms. The RAPD band patterns of the stem
-infecting form isolates were almost identical, while those of the lea
f-infecting isolates showed up to 29% variation.