L. Purnhauser, A METHOD FOR CROSSING NONSYNCHRONOUSLY FLOWERING PARENTS IN WHEAT, USING COLD-STORAGE OF THE FEMALE PARENT, Cereal Research Communications, 21(2-3), 1993, pp. 175-179
A simple method is described for making efficient hand crosses in whea
t (Triticum aestivum) parents with large differences in flowering date
s. It involves the cold storage of emasculated plants at 5-degrees-C b
etween emasculation and pollination. The seed set of cold-stored femal
es was high when spikes were pollinated 30 days after emasculation (DA
E). Female plants kept only in a greenhouse (30-degrees-C/20-degrees-C
day/night temperature) without cold storage showed a poor seed set wh
en pollinated 10 DAE, and the stigmas had completely lost their recept
ivity 15 DAE.