ESTIMATION OF BUNDLE-SHEATH CELL CONDUCTANCE IN C4 SPECIES AND O2 INSENSITIVITY OF PHOTOSYNTHESIS

Low conductance to CO2 of bundle sheath cells is required in C. photos ynthesis to maintain high [CO2] at the site of ribulose-1,5-bisphospha te carboxylase/oxygenase (Rubisco). Elevated [CO2] allows high CO2 ass imilation rates by this enzyme and prevents Rubisco oxygenase activity and O2 inhibition of carboxylation. Bundle sheath conductance to CO2 was estimated by chemically inhibiting phosphoenolpyruvate carboxylase and calculating the slope of the linear response of leaf CO2 uptake t o [CO2]. The inhibitor dichloro-2-dihydroxyphosphinoylmethyl-2-propeno ate was supplied to detached leaves of Panicum maximum, Panicum miliac eum, and Sorghum bicolor at 4 mm. Uptake of CO2 was measured at 210 mL L-1 O2 over the CO2 concentration range of 0.34 to 28 mL L-1. Without the inhibitor, CO2 uptake increased steeply at low [CO2] and saturate d at about 1 mL L-1. After inhibition, CO2 uptake was a linear functio n of [CO2] over much of the range tested. The slope of this CO2 respon se, taken as bundle sheath conductance, was 2.35, 1.96, and 1.13 mmol M-2 S-1 for P. maximum, P. miliaceum, and S. bicolor, respectively, on a leaf area basis. Conductance based on bundle sheath area was 0.76, 0.93, and 0.54 mmol M-2 s-1, respectively. Uptake Of CO2 by leaves of P. maximum supplied with the inhibitor was not affected by reduction o f [O2] from 210 to 20 mL L-1 over the range of [CO2] used. Because [CO 2] in bundle sheath cells of inhibited leaves is likely to be much low er than ambient, the lack of O2 sensitivity Of CO2 uptake cannot be as cribed to lack of O2 reaction with ribulose bisphosphate and is probab ly due to the low conductance of bundle sheath cells, especially at lo w ambient [CO2]. The likely result of reducing [O2] from 210 to 20 mL L-1 is to stimulate carboxylation of ribulose bisphosphate, thus furth er reducing [CO2] in bundle sheath cells and increasing CO2 diffusion to these cells from the mesophyll. However, the increase in diffusion is greatly limited by low conductance of the bundle sheath cell walls. Calculations based on estimated bundle sheath conductance show that c hanges in bundle sheath [CO2] of 0.085 to 0.5 mL L-1, which might be a ssociated with reduced [O2], would have a negligible effect on CO2 upt ake.