T. Ii et al., ELECTROSPRAY MASS-SPECTROMETRY OF PENTACOSA-SACCHARIDES OF BLOOD-GROUP I - ACTIVITY AND RELATED-COMPOUNDS, Organic mass spectrometry, 28(11), 1993, pp. 1340-1344
The structural characterization of large synthetic oligosaccharides an
d related glycoconjugates performed by the use of electrospray ionizat
ion mass spectrometry (ESIMS) is described. The largest molecules desc
ribed here are unprotected and protected pentaantennary pentacosasacch
arides 2 and 5 with average molecular masses of 4441 and 9416, respect
ively. The straight-chain neutral ceramide decasaccharide (d18:1/c24:0
) 6 and the disialoganglioside GD2 (d18:1/c24:0) 7 were also successfu
lly analyzed by ESIMS. In addition to exact mass determination, collis
ion-induced dissociation (CID) spectrometry of these compounds provide
d saccharide chain sequence information.