RAPID DIAGNOSIS OF CONTAGIOUS CAPRINE PLEUROPNEUMONIA IN PLEURAL FLUIDS BY IMMUNOBINDING ASSAY

Authors
GUERIN C THIAUCOURT F MADY V BREARD A LEFEVRE PC
Citation
C. Guerin et al., RAPID DIAGNOSIS OF CONTAGIOUS CAPRINE PLEUROPNEUMONIA IN PLEURAL FLUIDS BY IMMUNOBINDING ASSAY, Small ruminant research, 12(2), 1993, pp. 193-200
Citations number
NO
Categorie Soggetti
Agriculture Dairy & AnumalScience
Journal title
Small ruminant research → ACNP
ISSN journal
09214488
Volume
12
Issue
2
Year of publication
1993
Pages
193 - 200
Database
ISI
SICI code
0921-4488(1993)12:2<193:RDOCCP>2.0.ZU;2-L
Abstract
A diagnostic method for contagious caprine pleuropneumonia (CCPP), due to Mycoplasma sp. F38 is described. Mycoplasmal antigens were directl y detected in pleural fluids by an indirect immunobinding assay. The m ethod is rapid and sensitive but there exist cross-reactions within th e 'mycoides cluster, especially with Mycoplasma mycoides subsp. mycoid es LC (MmLC). Cross-reactivity may be minimized by testing samples aga inst different antisera. Immunobinding assay is proposed as a rapid di agnostic tool for field diagnosis of CCPP, and to increase the probabi lity of isolating Mycoplasma sp. F38.