Little is known about non-mycorrhizal endophytic fungi in tropical orc
hids; still less is known about how endophytes vary within and between
individual orchid plants. Fungal endophytes were isolated from roots
and leaves of epiphytic and lithophytic orchids in the genus Lepanthes
; seven species, from rainforests in Puerto Rico, were sampled. The en
dophytes observed most frequently were Xylaria species and Rhizoctonia
-like fungi, found in 29% of roots and 19% of leaves, and 45% of roots
and 31% of leaves, respectively. Five deuteromycete genera were also
isolated, occurring in 19% of roots and 43% of leaves (combined). At l
east nine species of Xylaria were found, with several species sometime
s occurring in a single plant. Differences between roots and leaves in
frequency of Xylaria and Rhizoctonia isolates were not significant, a
lthough differences among orchid species in number and types of endoph
ytes were. Heterogeneity of endophytes in single plants and plant orga
ns was greater than differences between species. Many Lepanthes specie
s are very restricted in distribution, and knowledge of their interact
ions with endophytes might be useful in species management.