SOMATIC-CELL MAPPING OF CONGLUTININ (CGN1) TO CATTLE SYNTENIC GROUP U29 AND FLUORESCENCE IN-SITU LOCALIZATION TO CHROMOSOME-28

A 260-bp genomic PstI fragment, which encodes a portion of the carbohy drate recognition domain, was used along with hybrid somatic cells to map the conglutinin gene (CGN1) to domestic cow (Bos taurus) syntenic group U29. In turn, a cosmid containing the entire bovine CGN1 was use d with fluorescence in situ hybridization to sublocalize this gene to cattle Chromosome (Chr) (BTA) 28 band 18. Since BTA 28 and several of the other small acrocentric autosomes of cattle are difficult to discr iminate, we have also chromosomally sublocalized CGN1 to the p arm of the lone biarmed autosome of the gaur (Bos gaurus). The use of the gau r 2/28 Robertsonian as a marker chromosome and our assignment of CCN1 to BTA 28 should help resolve some of the nomenclatural questions invo lving this cattle chromosome.