IMMUNOCHEMICAL ANALYSIS OF ESCHERICHIA COLI-EXPRESSED PRODUCTS OF VACCINIA VIRUS GENES I3 AND A2, STRAIN L-IVP

Authors
CHIKAEV NA MURAVLEV AI CHESHENKO NV GUTOROV VV MALYGIN EG
Citation
Na. Chikaev et al., IMMUNOCHEMICAL ANALYSIS OF ESCHERICHIA COLI-EXPRESSED PRODUCTS OF VACCINIA VIRUS GENES I3 AND A2, STRAIN L-IVP, Molecular biology, 27(2), 1993, pp. 173-176
Citations number
17
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
27
Issue
2
Year of publication
1993
Part
1
Pages
173 - 176
Database
ISI
SICI code
0026-8933(1993)27:2<173:IAOECP>2.0.ZU;2-9
Abstract
Genes I3 and A2 of the vaccinia virus (VV) strain L-IVP were cloned in bacterial expression vectors. Monospecific antisera were obtained to the products of these genes expressed in E. coli. By means of immunoch emical cross-analysis, two polypeptides of identical electrophoretic m obility comigrating with the major envelope protein p35 were detected in VV virion preparations. The major one is the product of gene I3, wh ereas the minor is encoded by gene A2.