ONE-TUBE, NESTED-PCR ASSAY FOR THE DETECTION OF TRICHOMONAS-VAGINALISIN VAGINAL DISCHARGES

Citation
Pr. Lin et al., ONE-TUBE, NESTED-PCR ASSAY FOR THE DETECTION OF TRICHOMONAS-VAGINALISIN VAGINAL DISCHARGES, Annals of tropical medicine and parasitology, 91(1), 1997, pp. 61-65
Citations number
14
Categorie Soggetti
Tropical Medicine",Parasitiology
ISSN journal
00034983
Volume
91
Issue
1
Year of publication
1997
Pages
61 - 65
Database
ISI
SICI code
0003-4983(1997)91:1<61:ONAFTD>2.0.ZU;2-C
Abstract
To shorten the time and improve the accuracy of diagnosis of vaginal t richomoniasis, a novel, one-tube, nested PCR, which targets a family o f 650-bp specific DNA repeats from the Trichomonas vaginalis genome, h as been developed. Samples were prepared by a rapid boiling method and the PCR products analysed by gel electrophoresis. A 290-bp DNA fragme nt was observed in all positive cases. No cross-reaction with any othe r pathogens, including the Pentatrichomonas hominis and Giardia lambli a used as controls, was found. Using the assay, one genome-equivalent of T. vaginalis in 20 mu l vaginal discharge can be detected and diagn osis can be made within 6 h. When 165 clinical specimens were examined by wet mount, culture and the PCR assay, 16 were found positive for T . vaginalis by both culture and PCR, whereas only nine of these 16 cas es were found to be positive by examination of met mounts. No PCR-nega tive cases were positive by wet mount or culture. This new assay appea rs to be a simple, rapid, accurate and sensitive method for the diagno sis of vaginal trichomoniasis.