ENANTIOMERIC LC SEPARATION OF CALCIUM-ANTAGONISTS ON PROTEIN-BASED CHIRAL STATIONARY PHASES

Three chiral calcium antagonist drugs, bepridil and two dihydropyridin e derivatives (nicardipine and REC 15/2375), have been successfully se parated within short retention times using either the al-acid glycopro tein chiral stationary phase (Chiral AGP) or the ovomucoid column (Ult ron ES-OVM). Aqueous buffer at defined pH is modified by the addition of an organic component (propan-2-ol, acetonitrile, ethanol) in order to modulate the retention properties of each system. The influence of pH and percentage of organic modifier on retention, selectivity, resol ution, and column performance are discussed for bepridil analyzed on C hiral AGP and for the two dihydropyridines (nicardipine and REC 15/237 5) analyzed on Ultron ES-OVM stationary phases. (C) Wiley-Liss Inc.