SEPARATION OF BENZO[ALPHA]PYRENE SULFATE ISOMERS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Authors
JOHNSON C GREENBERG A SANDER LC
Citation
C. Johnson et al., SEPARATION OF BENZO[ALPHA]PYRENE SULFATE ISOMERS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 753(2), 1996, pp. 201-206
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
753
Issue
2
Year of publication
1996
Pages
201 - 206
Database
ISI
SICI code
Abstract
A liquid chromatographic (LC) method has been developed for the separa tion of five isomers of benzo[a]pyrene sulfate. This method utilizes a polymeric C-18 column and isocratic elution with acetonitrile-water a nd triethylammonium acetate. Temperature, mobile phase composition, pH , type of C-18 stationary phase and triethylamine concentration were f ound to influence retention and selectivity, and separations were opti mized by proper choice of these parameters.