Cj. Shiff et al., THE RAPID MANUAL PARASIGHT(R)-F TEST - A NEW DIAGNOSTIC-TOOL FOR PLASMODIUM-FALCIPARUM INFECTION, Transactions of the Royal Society of Tropical Medicine and Hygiene, 87(6), 1993, pp. 646-648
Citations number
3
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
A rapid manual test for Plasmodium falciparum, the ParaSight(R)-F test
, has been used on a series of patients in a holoendemic malaria area
of coastal Tanzania. The test, which is an antigen capture test detect
ing trophozoite-derived histidine rich protein-II, is simple to perfor
m and provides a definitive answer in about 10 min. It requires no spe
cial equipment and is read using a single drop of blood. When compared
with 272 thick blood films examined microscopically by 2 observers an
d confirmed by the QBC(R) malaria test, the ParaSight(R)-F test had 88
.9% sensitivity and 87.5% specificity. Detectable antigenaemia in a gr
oup of 40 people declined following treatment with Fansidar(R) and by
10 d after treatment all but 4 individuals were antigen free. The rema
ining 4, although clear of peripheral parasitaemia, remained antigenae
mic for 14 d. The test shows great promise for rapid effective diagnos
is of P. falciparum in clinics and village health centres where there
is no facility for microscopy. Because of its accuracy and rapid actio
n it may even obviate the need for microscopical examination of blood
films to diagnose P. falciparum malaria.