PATTERNS OF PROTEIN-SYNTHESIS IN THE IMAGINAL DISKS OF DROSOPHILA-MELANOGASTER - A COMPARISON BETWEEN DIFFERENT DISKS AND STAGES

High-resolution two dimensional gel electrophoresis has been used to s tudy the patterns of protein synthesis in imaginal discs of Drosophila melanogaster. In this paper we first compare the patterns of protein synthesis in wing, haltere, leg 1, leg 2, leg 3 and eye antenna imagin al discs of late third instar larvae. We have detected only quantitati ve changes: differences in 17 proteins among the different imaginal di scs. In addition, we have analysed the variations in pattern of protei ns in the wing disc of the last larval stage and early pupae as well a s in wing discs cultured in vivo for 6 days. Variations in these patte rns affect more than 20% of the proteins and involve both qualitative and quantitative changes. Some of the changes may correspond to protei n phosphorylation. Correlations of these changes between discs and thr ough development are also discussed.