PERIPHERAL-BLOOD STEM-CELL COLLECTION WITH A BLOOD-CELL SEPARATOR

Forty-three patients with malignant nonmyeloid diseases underwent peri pheral blood stem cell collections on an apheresis system (Spectra, CO BE BCT, Lakewood, CO). Collections took place during the white cell (W BC) recovery phase following conditioning chemotherapy. One hundred tw o procedures were done after chemotherapy alone, and 72 procedures aft er chemotherapy plus granulocyte-colony-stimulating factor(G-CSF). Fou r centrifugal separation factors were tested. One and one-half patient blood volumes were processed in each procedure. The mean volume of th e collected component was 158 +/- 16 mL. After chemotherapy alone, the procedures provided a mean of 0.8 x 10(8) WBCs per kg and 2.3 x 10(4) colony-forming units-granulocyte macrophage (CFU-GM) per kg of recipi ent body weight. The mononuclear cell percentage in the components inc reased with the centrifugal separation factor from 85 to 96 percent. I n parallel, platelet contamination increased from 2.1 to 3.8 x 10(11). The collect hematocrit ranged from 1.0 to 2.5 percent (0.01-0.025). T he collection efficiency for mononuclear cells and CFU-GM also increas ed with the centrifugal separation factors from 52 to 70 percent for m ononuclear cells and from 55 to 68 percent for CFU-GM. Collections per formed after G-CSF-stimulated mobilization were characterized by a hig her neutrophil contamination independent of centrifugal separation fac tor, which gave a mean mononuclear cell percentage of 64 percent in th e collected component. The average yield for these procedures was 2 x 10(8) WBCs per kg and 28 x 10(4) CFU-GM per kg. The collection efficie ncy for mononuclear cells Increased with the separation factor from 43 to 77 percent. The collection efficiency for CFU-GM was greater than 100 percent.