INHIBITION OF PDGF BB STIMULATED DNA-SYNTHESIS IN RAT AORTIC VASCULARSMOOTH-MUSCLE CELLS BY THE EXPRESSION OF A TRUNCATED PDGF-BETA RECEPTOR

Rat aortic vascular smooth muscle cells (VSMCs) were transfected with a vector encoding a truncated human PDGF beta receptor or the full-len gth human PDGF beta receptor. Cells stably expressing the truncated hu man PDGF beta receptor or the full-length human PDGF beta receptor wer e selected and the effect of PDGF BB on DNA synthesis in these cells w as studied. Cells expressing the full-length PDGF beta receptor entere d DNA synthesis normally, however, cells expressing the truncated PDGF beta receptor failed to enter DNA synthesis in response to PDGF BB. T hese data show that the mitogenic response of rat aortic vascular smoo th muscle cells to PDGF BB can be inhibited by the expression of a tru ncated PDGF beta receptor.