Pr. Kemp et al., INHIBITION OF PDGF BB STIMULATED DNA-SYNTHESIS IN RAT AORTIC VASCULARSMOOTH-MUSCLE CELLS BY THE EXPRESSION OF A TRUNCATED PDGF-BETA RECEPTOR, FEBS letters, 336(1), 1993, pp. 119-123
Rat aortic vascular smooth muscle cells (VSMCs) were transfected with
a vector encoding a truncated human PDGF beta receptor or the full-len
gth human PDGF beta receptor. Cells stably expressing the truncated hu
man PDGF beta receptor or the full-length human PDGF beta receptor wer
e selected and the effect of PDGF BB on DNA synthesis in these cells w
as studied. Cells expressing the full-length PDGF beta receptor entere
d DNA synthesis normally, however, cells expressing the truncated PDGF
beta receptor failed to enter DNA synthesis in response to PDGF BB. T
hese data show that the mitogenic response of rat aortic vascular smoo
th muscle cells to PDGF BB can be inhibited by the expression of a tru
ncated PDGF beta receptor.