MESSENGER-RNA AMPLIFICATION SYSTEM BY VIRAL REPLICASE IN TRANSGENIC PLANTS

Authors
MORI M KAIDO M OKUNO T FURUSAWA I
Citation
M. Mori et al., MESSENGER-RNA AMPLIFICATION SYSTEM BY VIRAL REPLICASE IN TRANSGENIC PLANTS, FEBS letters, 336(1), 1993, pp. 171-174
Citations number
26
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
336
Issue
1
Year of publication
1993
Pages
171 - 174
Database
ISI
SICI code
0014-5793(1993)336:1<171:MASBVR>2.0.ZU;2-3
Abstract
We have constructed transgenic tobacco plants (M1x2-FCP2IFN plants) ex pressing viral RNA replication genes of brome mosaic virus (BMV) and B MV RNA3 derivative (FCP2IFN) carrying the human gamma interferon (IFN- gamma) gene. In M1x2-FCP2IFN plants the RNA3 derivative expressed from the integrated cDNA was replicated and subgenomic RNA (i.e. mRNA of I FN-gamma) was produced by BMV replicase. The accumulation level of the mRNA of IFN-gamma was approximately 5-fold higher than that by the ca uliflower mosaic virus (CaMV) 35S RNA promoter. In addition IFN-gamma accumulated in M1x2-FCP2IFN plants.