SCANNING ELECTROCHEMICAL MICROSCOPY .23. REACTION LOCALIZATION OF ARTIFICIALLY PATTERNED AND TISSUE-BOUND ENZYMES

Authors
PIERCE DT BARD AJ
Citation
Dt. Pierce et Aj. Bard, SCANNING ELECTROCHEMICAL MICROSCOPY .23. REACTION LOCALIZATION OF ARTIFICIALLY PATTERNED AND TISSUE-BOUND ENZYMES, Analytical chemistry, 65(24), 1993, pp. 3598-3604
Citations number
26
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
65
Issue
24
Year of publication
1993
Pages
3598 - 3604
Database
ISI
SICI code
0003-2700(1993)65:24<3598:SEM.RL>2.0.ZU;2-Q
Abstract
The scanning electrochemical microscope (SECM), operating in the feedb ack mode, was used to image localized surface reactions of redox enzym es at the micrometer level. Surfaces imaged with the SECM included glu cose oxidase immobilized within 8-mum-diameter pores of a filtration m embrane and individual whole mitochondria with active NADH cytochrome reductase enzymes in their outer membranes. Factors influencing enzyme image resolution and specificity are discussed.