THE QUANTIFICATION OF HYDROQUINONE, CATECHOL, PHENOL, 3-METHYLCATECHOL, SCOPOLETIN, M-CRESOL AND O-CRESOL IN INDOOR AIR SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY(P)
Ch. Risner, THE QUANTIFICATION OF HYDROQUINONE, CATECHOL, PHENOL, 3-METHYLCATECHOL, SCOPOLETIN, M-CRESOL AND O-CRESOL IN INDOOR AIR SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY(P), Journal of liquid chromatography, 16(18), 1993, pp. 4117-4140
A high performance liquid chromatography (HPLC) method was developed f
or the quantification of the phenolic compounds hydroquinone, catechol
, phenol, 3-methylcatechol, scopoletin, m+p-cresol and o-cresol in ind
oor air samples. Samples are collected on an 0.8 mum pore size mixed c
ellulose ester membrane (MCEM) followed by a silica gel Sep-Pak (SiOHS
P). The MCEM is extracted and the SiOHSP is eluted with 1 % acetic aci
d (HAc). The phenolic compounds were analyzed on a reverse-phase colum
n with fluorescence detection at selected excitation and emission wave
lengths specific to the compounds of interest. A mobile phase gradient
of 1 % HAc and 99 % acetonitrile (ACN) + 1 % HAc is used. The method
is reproducible with percent relative standard deviations (% RSD) rang
ing from 2.0 to 9.2 for the seven phenolic compounds. Percent recoveri
es are acceptable (88-98) with the exception of scopoletin (52) and p-
cresol (52). A comparison of tobacco versus wood smoke show that amoun
ts of these seven phenolic compounds vary widely with their source. A
relatively short sampling time (1-4 hours) is required and the procedu
re is capable of detecting < 0.3 mug m-3 for all compounds with the ex
ception of 3-methylcatechol with a detection limit of < 4.0 mug m-3.