CONDUCTING POLYMER-BASED AMPEROMETRIC ENZYME ELECTRODES - TOWARDS THEDEVELOPMENT OF MINIATURIZED REAGENTLESS BIOSENSORS

The development of reagentless enzyme electrodes implies the covalent binding of enzymes and redox mediators on the sensor surface to preven t contamination of the sample by sensor components. Additionally, mini aturization and mass production of enzyme electrodes require technique s for biosensor assembling avoiding manual deposition procedures. The electrochemical deposition of conducting polymer layers, e.g., polypyr role, occurs at the surface of an electrode independent of its size an d form. After the functionalization of conducting polymer films, elect rode surfaces can be obtained which are suitable for the binding of en zymes or redox mediators. New reagentless enzyme electrodes can be obt ained by electrochemical copolymerization of '-bipyridine)2(3-{pyrrol- 1-ylmethyl}pyridine)Cl]+, N-methylpyrrole and pyrrole-modified glucose oxidase leading to ternary copolymers simultaneously integrating redo x relays and enzyme activity.