SYNCHRONIZED CYCLIC CAPILLARY ELECTROPHORESIS - A NOVEL-APPROACH TO ION SEPARATIONS IN SOLUTION

Authors
BURGGRAF N MANZ A EFFENHAUSER CS VERPOORTE E DEROOIJ NF WIDMER HM
Citation
N. Burggraf et al., SYNCHRONIZED CYCLIC CAPILLARY ELECTROPHORESIS - A NOVEL-APPROACH TO ION SEPARATIONS IN SOLUTION, HRC. Journal of high resolution chromatography, 16(10), 1993, pp. 594-596
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
HRC. Journal of high resolution chromatography → ACNP
ISSN journal
09356304
Volume
16
Issue
10
Year of publication
1993
Pages
594 - 596
Database
ISI
SICI code
0935-6304(1993)16:10<594:SCCE-A>2.0.ZU;2-R
Abstract
A novel concept for repeated column switching in capillary electrophor esis is presented. Theoretical considerations predict high plate numbe rs per volt. Using micromachining techniques, a planar glass structure has been fabricated, which contains four capillaries of 20 mm length arranged in a square. Laser fluorescence detection gave a cycle time o f 70 s for 2.5 kV applied. After five cycles a theoretical plate numbe r of 40,000 was reached.